Pharm Chem 219: Mass Spectrometry-based Proteomics

Spring 2025

Proteomics is broadly concerned with gaining new knowledge of protein composition at the cellular, sub-cellular, or protein-complex level, and how protein functions are modulated by posttranslational dynamics, e.g. phosphorylation pathways or ubiquitylome/proteasome homeostasis, or how they are altered in disease states. Mass spectrometry is the technology of choice for detecting this information due to its ability to analyze complex samples for both discovery and quantification.

Mini-Course Description: This course will focus on the practical aspects (i.e. experimental and mass spectral interpretation) involved in the identification of proteins and their covalent modifications. It will cover the fundamental principles of currently important mass spectrometry instrument platforms. It will provide an overview of key scientific problems that are being tackled and solved at the protein-level relevant to cell function/dysfunction; the detection and assignment of protein posttranslational modifications; and studies of protein or modification dynamics using relative quantitation. It will also cover studies of the architecture of protein complexes and machines.

Participants will perform analysis of supplied datasets to learn how to evaluate results, and time will be scheduled at the end of the course for presentation and discussion of results obtained.

Schedule:
April 21 – May 9, 2025; Mo and Th 1-3pm
Mission Bay Campus, BH-413
Student presentations will be scheduled during the course.

Lecturers